The salt induced flocculation phenomenon is often proposed as a major mechanism influencing the deposition of the suspended matter near the mouth of major rivers. However, depending on the particular mixing conditions between fresh water and marine water and on suspended solids (SS) reactivity, salt induced aggregation may be a minor factor controlling fast deposition of SS. This work combines field studies and laboratory experiments in order to assess the importance of salt induced flocculation in the case of the saltwedge estuary of the Rhône river. Sampling of the mixing zone has been performed in contrasted hydrodynamic conditions from a low water discharge period (500 m3 s−1) to a small flood event (2400 m3 s−1) for particle counting and salinity measurements. Through laboratory experiments, it is shown that the Rhône river particulate matter has a poor average reactivity regarding salt induced flocculation. Considering the hydrodynamics of the estuary, we show that the evolution of the concentration of larger particles (>5 μm) can be explained by settling and dilution. In contrast, the smallest measured fraction (2–5 μm) shows a more complex behavior and settling processes alone cannot explain the observed particle concentrations during the field studies. Four hypotheses are discussed for explaining the 2–5 μm particle concentration evolutions.