HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Flavonoids, cytotoxic, antioxidant and antibacterial activities of Evax pygmaea

Abstract : Context: Phytochemical study and biological potential of Evax pygmaea (L.) Brot. (Asteraceae) are reported for the first time. Objective: To identify the secondary metabolites of Evax pygmaea and to determine its antioxidant, antibacterial and cytotoxic activities. Materials and methods: Dried aerial parts (1 kg) were macerated in 70% MeOH (5 L) during 72 h. The concentrated hydromethanolic extract was subjected to extractions with chloroform (3 × 300 mL), ethyl acetate (3 × 300 mL) and n-butanol (3 × 300 mL), successively. VLC of combined ethyl acetate (EAEP) and n-butanol (BEP) fractions was followed by column purifications. Antioxidant activity was investigated using DPPH, CUPRAC, and metal chelating, β-carotene/linoleic acid and ABTS assays. Agar method was used in the antibacterial study. Cytotoxic activity was determined by Brine shrimp lethality test in DMSO and ethanol, at varying concentrations (2, 1 and 0.2%) and (1, 0.2 and 0.1%) successively. Results: Quercetin (1), isorhamnetin 3-O-β-d-xyloside (2), isorhamnetin 3-O-β-d-glucoside (3), quercetin 3-O-β-d-glucoside (4), quercetin 7-O-β-D-glucoside (5), patuletin 3-O-β-d-glucoside (6) were isolated from for the first time from Evax genus. The EAEP was the most active in ABTS (IC50: <3.125 μg/mL) assay whereas the BEEP exhibited the highest activity in the β-carotene/linoleic acid assay (IC50: <3.125 μg/mL). The EAEP and BEP exhibited good antibacterial activity (MIC: 40–80 µg/mL). The plant did not show any toxicity (LD50>80 µg/mL). Discussion and conclusions: Six flavonoids were isolated for the first time from Evax pygmaea which exhibited good antioxidant and antibacterial activities.
Document type :
Journal articles
Complete list of metadata

Contributor : Abdulmagid Alabdul Magid Connect in order to contact the contributor
Submitted on : Friday, November 5, 2021 - 1:56:55 PM
Last modification on : Friday, March 18, 2022 - 4:30:04 PM
Long-term archiving on: : Sunday, February 6, 2022 - 6:53:25 PM


Flavonoids cytotoxic antioxida...
Publisher files allowed on an open archive




Assia Khalfallah, Djemaa Berrehal, Chawki Bensouici, Ahmed Kabouche, Zahia Semra, et al.. Flavonoids, cytotoxic, antioxidant and antibacterial activities of Evax pygmaea. Pharmaceutical biology, Taylor & Francis, 2017, 55 (1), pp.2292 - 2296. ⟨10.1080/13880209.2017.1405997⟩. ⟨hal-01834125⟩



Record views


Files downloads