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13 C NMR and LC-MS Profiling of Stilbenes from Elicited Grapevine Hairy Root Cultures

Abstract : Resveratrol and related oligostilbenes are defense molecules produced by grapevine in response to stresses including various elicitors or signal molecules. Together with their prominent role in planta, these compounds have been the center of much attention in recent decades due to their pharmacological properties. The cost-effective production of resveratrol derivatives such as viniferins or more structurally complex stilbene oligomers remains a challenging task. In this study, the chemical diversity of stilbenes produced by Vitis vinifera Pinot Noir hairy roots was investigated after elicitation for 4 days with a mixture of methyl jasmonate (100 μM) and cyclodextrins (50 mM). Two crude extracts obtained from the culture medium and from the hairy roots were fractionated by centrifugal partition chromatography. The fractions were chemically investigated by two complementary identification approaches involving a 13C NMR-based dereplication method and liquid chromatography coupled to mass spectrometry (LC-MS). In total, groups of 21 and 18 molecules, including flavonoids and stilbenes, were detected in the culture medium and root extracts, respectively. These included resveratrol monomers, dimers, trimers, and a tetramer, thus highlighting the ability of elicited hairy root culture systems to synthesize a wide diversity of secondary metabolites of pharmaceutical significance. The main compounds were unambiguously identified as trans-resveratrol, ε-viniferin, trans-piceatannol, pallidol, scirpusin A, eriodictyol, naringenin, vitisin B, and maackin.
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Contributor : Jean-Marc Nuzillard Connect in order to contact the contributor
Submitted on : Thursday, October 25, 2018 - 2:47:31 PM
Last modification on : Tuesday, December 14, 2021 - 9:04:03 AM




Leo-Paul Tisserant, Jane Hubert, Michelle Lequart-Pillon, Nicolas Borie, Nicolas Maurin, et al.. 13 C NMR and LC-MS Profiling of Stilbenes from Elicited Grapevine Hairy Root Cultures. Journal of Natural Products, American Chemical Society, 2016, 79 (11), pp.2846 - 2855. ⟨10.1021/acs.jnatprod.6b00608⟩. ⟨hal-01904968⟩



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