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Journal articles
Emergence of Antimicrobial-Resistant Escherichia coli of Animal Origin Spreading in Humans
David Skurnik
1, 2, 3
Olivier Clermont
2
Thomas Guillard
1
Adrien Launay
2
Olga Danilchanka
1
Stéphanie Pons
1
Laure Diancourt
4
François Lebreton
1
Kristina Kadlec
5
Damien Roux
1, 2
Deming Jiang
Sara Dion
2
Hugues Aschard
1
Maurice Denamur
Colette Cywes-Bentley
Stefan Schwarz
Olivier Tenaillon
2
Antoine Andremont
2
Bertrand Picard
John Mekalanos
1
Sylvain Brisse
4
Erick Denamur
2
David Skurnik 1, 2, 3
Author
Olivier P Clermont 2
Author
Thomas Guillard 1
Author IdHAL : thomas-guillard ResearcherId : http://www.researcherid.com/rid/G-4065-2013 IdRef : https://www.idref.fr/118182307 ORCID : https://orcid.org/0000-0002-3795-0398
Adrien Launay 2
Author
Olga Danilchanka 1
Author
Stéphanie Pons 1
Author
Laure Diancourt 4
Author IdHAL : laure-diancourt ORCID : https://orcid.org/0000-0002-9670-3274
François Lebreton 1
Author
Olivier Tenaillon 2
Author
Antoine Andremont 2
Author
Bertrand Picard
Author
John Mekalanos 1
Author
Sylvain Brisse 4
Author IdHAL : sylvain-brisse ORCID : https://orcid.org/0000-0002-2516-2108
Erick P Denamur 2
Author
1
HMS - Harvard Medical School [Boston] (25 Shattuck Street Boston, MA 02115 - United States)
2
IAME (UMR_S_1137 / U1137) - Infection, Anti-microbiens, Modélisation, Evolution (Faculté de médecine Paris Diderot Paris 7 - site Bichat - 16 rue Henri Huchard 75890 Paris Cedex 18 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1137 (101, rue de Tolbiac, 75013 Paris - France)
- UP13 - Université Paris 13 (France)
- UPD7 - Université Paris Diderot - Paris 7 : UMR_S_1137 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
- USPC - Université Sorbonne Paris Cité (Université Sorbonne Paris Cité | 100-104, avenue de France | 75013 Paris - France)
3
CNR « Résistance dans les flores commensales » (Paris - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- AP-HP - Hôpital Bichat - Claude Bernard [Paris] (46 Rue Henri Huchard, 75018 Paris - France)
4
PF8 - Génotypage des Pathogènes et Santé Publique (Plate-forme) (25-28 rue du Docteur Roux, F-75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
5
ING - Institute of Farm Animal Genetics (Institut für Nutztiergenetik
Höltystr. 10
31535 Neustadt
- Germany)
- FLI - Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health Südufer 10 D-17493 Greifswald - Insel Riems - Germany)
Abstract : In the context of the great concern about the impact of human activities on the environment, we studied 403 commensal Escherichia coli/Escherichia clade strains isolated from several animal and human populations that have variable contacts to one another. Multilocus sequence typing (MLST) showed a decrease of diversity 1) in strains isolated from animals that had an increasing contact with humans and 2) in all strains that had increased antimicrobial resistance. A specific B1 phylogroup clonal complex (CC87, Institut Pasteur schema nomenclature) of animal origin was identified and characterized as being responsible for the increased antimicrobial resistance prevalence observed in strains from the environments with a high human-mediated antimicrobial pressure. CC87 strains have a high capacity of acquiring and disseminating resistance genes with specific metabolic and genetic determinants as demonstrated by high-throughput sequencing and phenotyping. They are good mouse gut colonizers but are not virulent. Our data confirm the predominant role of human activities in the emergence of antimicrobial resistance in the environmental bacterial strains and unveil a particular E. coli clonal complex of animal origin capable of spreading antimicrobial resistance to other members of microbial communities.
Document type :
Journal articles
Complete list of metadata
https://hal.univ-reims.fr/hal-02453700
Contributor : Valerian Dormoy Connect in order to contact the contributor
Submitted on : Friday, January 24, 2020 - 8:06:43 AM
Last modification on : Tuesday, October 19, 2021 - 10:26:44 PM
Contributor : Valerian Dormoy Connect in order to contact the contributor
Submitted on : Friday, January 24, 2020 - 8:06:43 AM
Last modification on : Tuesday, October 19, 2021 - 10:26:44 PM
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Identifiers
- HAL Id : hal-02453700, version 1
- DOI : 10.1093/molbev/msv280
- PUBMED : 26613786
Collections
URCA | P3CELL | IAME | UNIV-PARIS13 | UNIV-PARIS7 | USPC | PASTEUR | SORBONNE-PARIS-NORD | UNIV-PARIS | UP-SANTE
Citation
David Skurnik, Olivier Clermont, Thomas Guillard, Adrien Launay, Olga Danilchanka, et al.. Emergence of Antimicrobial-Resistant Escherichia coli of Animal Origin Spreading in Humans. Molecular Biology and Evolution, Oxford University Press (OUP), 2016, 33 (4), pp.898-914. ⟨10.1093/molbev/msv280⟩. ⟨hal-02453700⟩
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