This article aims at investigating the effects of hygrothermal aging on the damage mechanisms of short white Hemp Fiber Reinforced Polypropylene (HFRP) composites with various fiber contents (10, 20, 30, and 40 wt%). Injected molded specimens were subjected to hygrothermal aging with a relative humidity of 80% and two temperatures, 25 and 50°C. The water absorption and its effect on tensile properties of HFRP composites were investigated. The Acoustic Emission (AE) technique combined with scanning electron microscopy observations was used to identify microstructural damage events leading to overall failure of the HFRP composites. This identification according to hemp fiber content and hygrothermal aging was made with an unsupervised method based on a statistical multivariable analysis (k-means algorithm). The AE results indicate that the quality of fiber-matrix interface plays a major role in the damage process of HFRP composites, shown by the number of AE signals induced by the interface failure and their amplitude ranges.