Ion exchange centrifugal partition chromotography: an innovative tool for bioactive peptide capture in complex protein hydrolyzate - Université de Reims Champagne-Ardenne
Poster De Conférence Année : 2012

Ion exchange centrifugal partition chromotography: an innovative tool for bioactive peptide capture in complex protein hydrolyzate

Résumé

Alfalfa (Medicago sativa) is a common feed, with a high protein contain of good nutritional value like RuBisCO (Ribulose-I ,5-biphosphatase). RuBisCO hydrolysis using Delvolase® leads to a complex mixture ofpeptides (average MW= 600 g.mol-1), which exhibits an opioid effect. Among this mixture, one dipeptide VW is an angiotensin-converting enzyme inhibitor. In order to develop nutraceutical anti-hypertensive product, VW content of 0.3% must be improved. Centrifugal Partition Chromatography (CPC) is a support free liquid/liquid chromatography process, which has demonstrated high interest for analyte capture inside complex matrix, typically natural product extracts or crude hydrolyzates, especially in ion exchange mode. Here is described a new ion exchange CPC process designed for peptide capture and applied to RuBisCO hydrolyzate. Enriched fractions were obtained, containing 11% of VW, with a high recovery rate of 97%.

Domaines

Pharmacologie
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Dates et versions

hal-02746703 , version 1 (03-06-2020)

Identifiants

  • HAL Id : hal-02746703 , version 1
  • PRODINRA : 177591
  • WOS : 000307042801499

Citer

Leslie Boudesocque, Romain Kapel, Pascal Dhulster, Ivan Marc, Jean-Hugues Renault. Ion exchange centrifugal partition chromotography: an innovative tool for bioactive peptide capture in complex protein hydrolyzate. 2012 International Congress on Natural Products Research, Jul 2012, New York, United States. Georg Thieme Verlag, Planta Medica, 78 (11), 2012. ⟨hal-02746703⟩
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