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A new gallium complex inhibits tumor cell invasion and matrix metalloproteinase MMP-14 expression and activity

Abstract : In this study, we investigated the effect of [N-(5-chloro-2-hydroxyphenyl)-l-aspartato] chlorogallate (GS2), a new water soluble gallium complex, on cell invasion and on the expression and activity of matrix metalloproteinases (MMPs) in human metastatic HT-1080 fibrosarcoma and MDA-MB 231 breast carcinoma cells. The effect on cell invasion was studied using a modified Boyden chamber coated with a type-I collagen. We analyzed the effect of GS2 on MMP-2, MMP-9, and MMP-14 via zymography and enzymatic assay using high affinity fluorogenic substrates. The expression of MMP mRNA was analyzed via qRT-PCR. GS2 induced a decrease in cell invasion. A dose-dependent inhibition effect was observed on the activities of MMP-2, MMP-9, and MMP-14 with the IC50 values of 168, 82, and 20 μM, respectively. A decrease in the expression of MMP-14 mRNA was observed in both cell lines, whereas the expression of MMP-2 and MMP-9 mRNA was decreased only in the MDA-MB231 cells. Data obtained for the expression of MMP-14 mRNA were confirmed via Western blotting. In fact, MMP-14 expression was decreased in the presence of GS2. Overall, these data show that GS2 is a promising compound for anti-invasive and anticancer therapy.
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https://hal.univ-reims.fr/hal-02929237
Contributor : Brigitte Perron <>
Submitted on : Thursday, September 3, 2020 - 11:57:52 AM
Last modification on : Wednesday, September 16, 2020 - 5:48:47 PM

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Ahmed Mohsen, Philippe Collery, Roselyne Garnotel, Bertrand Brassart, Nicolas Etique, et al.. A new gallium complex inhibits tumor cell invasion and matrix metalloproteinase MMP-14 expression and activity. Metallomics, Royal Society of Chemistry, 2017, 9 (8), pp.1176-1184. ⟨10.1039/c7mt00049a⟩. ⟨hal-02929237⟩

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