Skip to Main content Skip to Navigation
Journal articles

The Zebra Mussel (Dreissena polymorpha) as a Model Organism for Ecotoxicological Studies: A Prior 1H NMR Spectrum Interpretation of a Whole Body Extract for Metabolism Monitoring

Abstract : The zebra mussel (Dreissena polymorpha) represents a useful reference organism for the ecotoxicological study of inland waters, especially for the characterization of the disturbances induced by human activities. A nuclear magnetic resonance (NMR)-based metabolomic approach was developed on this species. The investigation of its informative potential required the prior interpretation of a reference 1 H NMR spectrum of a lipid-free zebra mussel extract. After the extraction of polar metabolites from a pool of whole-body D. polymorpha powder, the resulting highly complex 1D 1 H NMR spectrum was interpreted and annotated through the analysis of the corresponding 2D homonuclear and heteronuclear NMR spectra. The spectrum interpretation was completed and validated by means of sample spiking with 24 commercial compounds. Among the 238 detected 1 H signals, 53% were assigned, resulting in the identification of 37 metabolites with certainty or high confidence, while 5 metabolites were only putatively identified. The description of such a reference spectrum and its annotation are expected to speed up future analyses and interpretations of NMR-based metabolomic studies on D. polymorpha and to facilitate further explorations of the impact of environmental changes on its physiological state, more particularly in the context of large-scale ecological and ecotoxicological studies.
Complete list of metadatas

Cited literature [64 references]  Display  Hide  Download

https://hal.univ-reims.fr/hal-02934872
Contributor : Jean-Marc Nuzillard <>
Submitted on : Thursday, September 10, 2020 - 8:05:51 AM
Last modification on : Wednesday, October 14, 2020 - 4:13:37 AM

File

metabolites-10-00256-v3.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution - NoDerivatives 4.0 International License

Identifiers

Citation

Sophie Martine Prud’homme, Younes Mohamed Ismail Hani, Neil Cox, Guy Lippens, Jean-Marc Nuzillard, et al.. The Zebra Mussel (Dreissena polymorpha) as a Model Organism for Ecotoxicological Studies: A Prior 1H NMR Spectrum Interpretation of a Whole Body Extract for Metabolism Monitoring. Metabolites, MDPI, 2020, 10 (6), pp.256. ⟨10.3390/metabo10060256⟩. ⟨hal-02934872⟩

Share

Metrics

Record views

66

Files downloads

36