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Journal articles

Evaluation of lumican effects on morphology of invading breast cancer cells, expression of integrins and downstream signaling

Abstract : The small leucine-rich proteoglycan lumican regulates estrogen receptors (ERs)-associated functional properties of breast cancer cells, expression of matrix macromolecules, and epithelial-to-mesenchymal transition. However , it is not known whether the ER-dependent lumican effects on breast cancer cells are related to the expression of integrins and their intracellular signaling pathways. Here, we analyzed the effects of lumican in three breast cancer cell lines: the highly metastatic ERb-positive MDA-MB-231, cells with the respective ERb-suppressed (shERbMDA-MB-231), and lowly invasive ERa-positive MCF-7/c breast cancer cells. Scanning electron microscopy, confocal microscopy, real-time PCR, western blot, and cell adhesion assays were performed. Lumican effects on breast cancer cell morphology were also investigated in 3-dimensional collagen cultures. Lumican treatment induced cell-cell contacts and cell grouping and inhibited microvesicles and microvilli formation. The expression of the cell surface adhesion receptor CD44, its isoform and variants, hyaluronan (HA), and HA synthases was also investigated. Lumican inhibited the expression of CD44 and HA synthases, and its effect on cell adhesion revealed a major role of a1, a2, a3, aVb3, and aVb5 integrins in MDA-MB-231 cells, but not in MCF-7/c cells. Lumican upregulated the expression of a2 and b1 integrin subunits both in MDA-MB-231 and in shERbMDA-MB-231 as compared to MCF-7/c cells. Downstream signaling pathways for inte-grins, such as FAK, ERK 1/2 MAPK 42/44, and Akt, were found to be downregulated by lumican. Our data shed light to the molecular mechanisms responsible for the anticancer activity of lumican in invasive breast cancer. Abbreviations As, anti-sense; CD44, cluster of differentiation 44; CD44s, CD44
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Contributor : Jean-Marc Ferez Connect in order to contact the contributor
Submitted on : Tuesday, November 17, 2020 - 10:59:09 AM
Last modification on : Wednesday, March 9, 2022 - 2:00:06 PM
Long-term archiving on: : Thursday, February 18, 2021 - 7:02:20 PM


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Konstantina Karamanou, Marco Franchi, Maurizio Onisto, Alberto Passi, Demitrios Vynios, et al.. Evaluation of lumican effects on morphology of invading breast cancer cells, expression of integrins and downstream signaling. FEBS Journal, Wiley, 2020, 287, pp.4862 - 4880. ⟨10.1111/febs.15289⟩. ⟨hal-02986565⟩



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