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Infrared Microspectroscopy and Imaging Analysis of Inflammatory and Non-Inflammatory Breast Cancer Cells and Their GAG Secretome

Abstract : Glycosaminoglycans (GAGs)/proteoglycans (PGs) play a pivotal role in the metastasis of inflammatory breast cancer (IBC). They represent biomarkers and targets in diagnosis and treatment of different cancers including breast cancer. Thus, GAGs/PGs could represent potential prognostic/diagnostic biomarkers for IBC. In the present study, non-IBC MDA-MB-231, MCF7, SKBR3 cells and IBC SUM149 cells, as well as their GAG secretome were analyzed. The latter was measured in toto as dried drops with high-throughput (HT) Fourier Transform InfraRed (FTIR) spectroscopy and imaging. FTIR imaging was also employed to investigate single whole breast cancer cells while synchrotron-FTIR microspectroscopy was used to specifically target their cytoplasms. Data were analyzed by hierarchical cluster analysis and principal components analysis. Results obtained from HT-FTIR analysis of GAG drops showed that the inter-group variability enabled us to delineate between cell types in the GAG absorption range 1350-800 cm −1. Similar results were obtained for FTIR imaging of GAG extracts and fixed single whole cells. Synchrotron-FTIR data from cytoplasms allowed discrimination between non-IBC and IBC. Thus, by using GAG specific region, not only different breast cancer cell lines could be differentiated, but also non-IBC from IBC cells. This could be a potential diagnostic spectral marker for IBC detection useful for patient management.
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Contributor : Jean-Marc Ferez Connect in order to contact the contributor
Submitted on : Tuesday, November 17, 2020 - 11:28:56 AM
Last modification on : Wednesday, November 3, 2021 - 3:51:51 PM
Long-term archiving on: : Thursday, February 18, 2021 - 7:08:41 PM


Mohamed et al, molecules-25-04...
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Distributed under a Creative Commons Attribution 4.0 International License




Hossam Taha Mohamed, Valérie Untereiner, Gianfelice Cinque, Sherif Abdelaziz Ibrahim, Martin Götte, et al.. Infrared Microspectroscopy and Imaging Analysis of Inflammatory and Non-Inflammatory Breast Cancer Cells and Their GAG Secretome. Molecules, MDPI, 2020, 25 (18), pp.4300. ⟨10.3390/molecules25184300⟩. ⟨hal-02986578⟩



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