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, Human tissues were transferred to our laboratory in RPMI 1640 medium supplemented with 20 mM HEPES (Gibco) and antibiotics (200 UI/ml penicillin, 200 µg/ml streptomycin; Gibco)

, Figure 5?figure supplement 2. Effects of Silencing of MLYCD in RPMI-8226 cells.

A. D0, A. D7, A. D15, A. D25, and A. D35, Switzerland) and seeded (5x10 4 cells per membrane) on type IV collagen (Sigma Aldrich)-coated porous 12 mm from the upper compartment (ALI D0) and the epithelium was allowed to differentiate at the ALI with supplemented BEGM/DMEM/F12 of 5% CO2. In some experiments, the ALI medium was supplemented with a cocktail of pro-inflammatory cytokines called cytomix from ALI D0 to ALI D35, Nasal HAE cells (P0) were suspended in CnT17 medium (CELLnTEc

, CF and CF HAE cells were plated on type IV collagen-coated plastic flasks for cell proliferation in CnT17 medium. When confluent, the cells were detached by tryspine/EDTA (Gibco) treatment and called cells at passage P1 (P1)

. Zoeterwoude, The Netherlands), frozen in liquid nitrogen and stored at -80°C until use for histology and immunohistochemistry

, Seven-micrometer frozen sections were cut with a microtome, collected on superfrost slides (Thermo Scientific, Braunschweig, Germany), and either stained with hematoxylin-eosin using Rapid-Chrome Frozen Sections Staining Kit, ) and mounted in Eukitt (Electron Microscopy Sciences

K. Monteith, Phosphate-buffered Saline (PBS) v1 (protocols.io.p4rdqv6), 2018.

, BSA 10% for 30 minutes at room temperature (RT) to prevent unspecific bindings. ALI D35 sections were incubated with mouse anti-human CK13 (dilution 1/1000), mouse anti-MUC

, Figure 5?figure supplement 1. Proportions of anti-prM antibodies from both D2VLP immunization groups were measured using an epitope-blocking ELISA., vol.7, p.15

, diluted in PBS-BSA 3 % overnight at 4°C. After washes with PBS at RT, sections were blocked again with PBS-BSA 10 % for 30 minutes at RT, then incubated with the goat anti-mouse Alexa Fluor 488 secondary antibodies diluted in PBS/BSA 3% (dilution 1/200) for 60 minutes at RT, washed with PBS, and D35 culture sections with mouse anti-human Ki67 antibodies (dilution 1/10)

, MDCK cells were infected with influenza A virus and were fixed at 7 h postinfection. The cells were subjected to indirect immunostaining with anti-alpha-tubulin monoclonal antibody (clone DM1A, Sigma-Aldrich) and Alexa Fluor 488-conjugated anti-mouse Ig., Microbiology and Immunology, vol.59, issue.2, pp.i-i, 2015.

. Fab, Fab fragments

J. Immunoresearch, PA) to block free sites of mouse anti-MUC-5B antibodies, and finally stained with anti-MUC-5AC antibodies followed by incubation with Alexa Fluor 594-goat anti-mouse antibodies (dilution 1/200 in PBS/BSA 3%; Molecular Probe

, Author response image 1. Anti-DDR1 mAbs inhibit collagen-induced DDR1 clustering.

, Figure 8?figure supplement 1. 5-Ethynyl-2?-deoxyuridine (EdU) incorporation assay.