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Molecular characterization and epidemiology of cefoxitin resistance among Enterobacteriaceae lacking inducible chromosomal ampC genes from hospitalized and non-hospitalized patients in Algeria: description of new sequence type in Klebsiella pneumoniae isolates

Abstract : tIn this study, 922 consecutive non-duplicate clinical isolates of Enterobacteriaceae obtainedfrom hospitalized and non-hospitalized patients at Bejaia, Algeria were analyzed forAmpC-type -lactamases production. The ampC genes and their genetic environmentwere characterized using polymerase chain reaction (PCR) and sequencing. Plasmidincompatibility groups were determined by using PCR-based replicon typing. Phylogeneticgrouping and multilocus sequence typing were determined for molecular typing of theplasmid-mediated AmpC (pAmpC) isolates.Of the isolates, 15 (1.6%) were identified as AmpC producers including 14 CMY-4-producing isolates and one DHA-1-producing Klebsiella pneumoniae. All AmpC-producingisolates co-expressed the broad-spectrum TEM-1 -lactamase and three of them co-produced CTX-M and/or SHV-12 ESBL. Phylogenetic grouping and virulence genotyping ofthe E. coli isolates revealed that most of them belonged to groups D and B1. Multilocussequence typing analysis of K. pneumoniae isolates identified four different sequence types(STs) with two new sequences: ST1617 and ST1618. Plasmid replicon typing indicatesthat blaCMY-4gene was located on broad host range A/C plasmid, while LVPK repliconwas associated with blaDHA-1. All isolates carrying blaCMY-4displayed the transposon-likestructures ISEcp1/ISEcp1-blaCMY-blc-sugE.Our study showed that CMY-4 was the main pAmpC in the Enterobacteriaceae isolates inAlgeria.©
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https://hal.univ-reims.fr/hal-03011840
Contributor : Valerian Dormoy <>
Submitted on : Tuesday, February 9, 2021 - 12:05:00 PM
Last modification on : Thursday, February 11, 2021 - 11:40:09 AM

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Alima Gharout-Sait, Abdelaziz Touati, Thomas Guillard, Lucien Brasme, Christophe de Champs. Molecular characterization and epidemiology of cefoxitin resistance among Enterobacteriaceae lacking inducible chromosomal ampC genes from hospitalized and non-hospitalized patients in Algeria: description of new sequence type in Klebsiella pneumoniae isolates. Brazilian Journal of Infectious Diseases, Elsevier, 2015, 19 (2), pp.187-195. ⟨10.1016/j.bjid.2014.12.001⟩. ⟨hal-03011840⟩

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