Rhamnolipids and their 3-(3-hydroxyalkanoyloxy)alkanoic acid precursors activate Arabidopsis innate immunity through two independent mechanisms - Université de Reims Champagne-Ardenne Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2021

Rhamnolipids and their 3-(3-hydroxyalkanoyloxy)alkanoic acid precursors activate Arabidopsis innate immunity through two independent mechanisms

Arvin Nickzad
  • Fonction : Auteur
Alexander Kutschera
  • Fonction : Auteur
Tim Gerster
  • Fonction : Auteur
Nicolas Borie
  • Fonction : Auteur
Corinna Dawid
  • Fonction : Auteur
Maude Cloutier
  • Fonction : Auteur
Jane Hubert
  • Fonction : Auteur
Christian Schmid
  • Fonction : Auteur
Marc Ongena
Jean-Hugues Renault
Arnaud Haudrechy
  • Fonction : Auteur
Thomas Hofmann
  • Fonction : Auteur
Cyril Zipfel
  • Fonction : Auteur
Charles Gauthier
  • Fonction : Auteur
Eric Déziel
  • Fonction : Auteur
Stefanie Ranf
  • Fonction : Auteur

Résumé

Plant innate immunity is activated upon perception of invasion pattern molecules by plant cell-surface immune receptors. Several bacteria of the genera Pseudomonas and Burkholderia produce rhamnolipids (RLs) from L-rhamnose and ( R )-3-hydroxyalkanoate precursors (HAAs). RL and HAA secretion is required to modulate bacterial swarming motility behavior. The bulb-type lectin receptor kinase LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION/S-DOMAIN-1-29 (LORE/SD1-29) mediates medium-chain 3-hydroxy fatty acid (mc-3-OH-FA) sensing in the plant Arabidopsis thaliana . Here, we show that the lipidic secretome from Pseudomonas aeruginosa comprising RLs, HAAs and mc-3-OH-FAs stimulates Arabidopsis immunity. HAAs, like mc-3-O-FAs, are sensed by LORE and induce canonical immune signaling and local resistance to plant pathogenic Pseudomonas infection. By contrast, RLs trigger an atypical immune response and resistance to Pseudomonas infection independent of LORE. Thus, the glycosyl moieties of RLs, albeit abolishing sensing by LORE, do not impair their ability to trigger plant defense. In addition, our results show that RL-triggered immune response is affected by the sphingolipid composition of the plasma membrane. In conclusion, RLs and their precursors released by bacteria can both be perceived by plants but through distinct mechanisms.

Dates et versions

hal-03115315 , version 1 (19-01-2021)

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Citer

Romain Schellenberger, Jérôme Crouzet, Arvin Nickzad, Alexander Kutschera, Tim Gerster, et al.. Rhamnolipids and their 3-(3-hydroxyalkanoyloxy)alkanoic acid precursors activate Arabidopsis innate immunity through two independent mechanisms. 2021. ⟨hal-03115315⟩

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