Rhamnolipids and their 3-(3-hydroxyalkanoyloxy)alkanoic acid precursors activate Arabidopsis innate immunity through two independent mechanisms - Archive ouverte HAL Access content directly
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Rhamnolipids and their 3-(3-hydroxyalkanoyloxy)alkanoic acid precursors activate Arabidopsis innate immunity through two independent mechanisms

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Arvin Nickzad
  • Function : Author
Alexander Kutschera
  • Function : Author
Tim Gerster
  • Function : Author
Nicolas Borie
  • Function : Author
Corinna Dawid
  • Function : Author
Maude Cloutier
  • Function : Author
Jane Hubert
  • Function : Author
Christian Schmid
  • Function : Author
Marc Ongena
  • Function : Author
Jean-Hugues Renault
Arnaud Haudrechy
  • Function : Author
Thomas Hofmann
  • Function : Author
Cyril Zipfel
  • Function : Author
Charles Gauthier
  • Function : Author
Eric Déziel
  • Function : Author
Stefanie Ranf
  • Function : Author

Abstract

Plant innate immunity is activated upon perception of invasion pattern molecules by plant cell-surface immune receptors. Several bacteria of the genera Pseudomonas and Burkholderia produce rhamnolipids (RLs) from L-rhamnose and ( R )-3-hydroxyalkanoate precursors (HAAs). RL and HAA secretion is required to modulate bacterial swarming motility behavior. The bulb-type lectin receptor kinase LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION/S-DOMAIN-1-29 (LORE/SD1-29) mediates medium-chain 3-hydroxy fatty acid (mc-3-OH-FA) sensing in the plant Arabidopsis thaliana . Here, we show that the lipidic secretome from Pseudomonas aeruginosa comprising RLs, HAAs and mc-3-OH-FAs stimulates Arabidopsis immunity. HAAs, like mc-3-O-FAs, are sensed by LORE and induce canonical immune signaling and local resistance to plant pathogenic Pseudomonas infection. By contrast, RLs trigger an atypical immune response and resistance to Pseudomonas infection independent of LORE. Thus, the glycosyl moieties of RLs, albeit abolishing sensing by LORE, do not impair their ability to trigger plant defense. In addition, our results show that RL-triggered immune response is affected by the sphingolipid composition of the plasma membrane. In conclusion, RLs and their precursors released by bacteria can both be perceived by plants but through distinct mechanisms.

Dates and versions

hal-03115315 , version 1 (19-01-2021)

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Romain Schellenberger, Jérôme Crouzet, Arvin Nickzad, Alexander Kutschera, Tim Gerster, et al.. Rhamnolipids and their 3-(3-hydroxyalkanoyloxy)alkanoic acid precursors activate Arabidopsis innate immunity through two independent mechanisms. 2021. ⟨hal-03115315⟩

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