Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding - Archive ouverte HAL Access content directly
Journal Articles Parasite Year : 2016

Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding

(1, 2) , (1) , (1) , (1, 3) , (1, 3)
1
2
3

Abstract

Detection of Toxoplasma gondii DNA in cat feces is considered indicative of the presence of T. gondii oocysts. This study aims to demonstrate that the high sensitivity of qPCR can lead to T. gondii DNA detection in cat feces in the absence of oocysts. A cat immune to toxoplasmosis was fed with a mouse experimentally infected with T. gondii. Detection of DNA of this parasite was performed by qPCR on feces passed: (i) on the day the cat ingested the infected prey; (ii) during the three previous days; and (iii) during the three following days. The kinetics of qPCR results are clearly not linked to oocyst shedding and this result demonstrates that qPCR can detect T. gondii DNA related to bradyzoites from an infected prey, in the absence of oocysts. Caution is thus recommended when interpreting T. gondii qPCR results for samples of cat feces.
Fichier principal
Vignette du fichier
parasite-23-29.pdf (323.41 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03128825 , version 1 (02-02-2021)

Identifiers

Cite

Marie-Lazarine Poulle, Marie-Amélie Forin-Wiart, Émilie Josse-Dupuis, Isabelle Villena, Dominique Aubert. Detection of Toxoplasma gondii DNA by qPCR in the feces of a cat that recently ingested infected prey does not necessarily imply oocyst shedding. Parasite, 2016, 23, pp.29. ⟨10.1051/parasite/2016029⟩. ⟨hal-03128825⟩
46 View
37 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More