Major 5′terminally deleted enterovirus populations modulate type I IFN response in acute myocarditis patients and in human cultured cardiomyocytes - Université de Reims Champagne-Ardenne Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2020

Major 5′terminally deleted enterovirus populations modulate type I IFN response in acute myocarditis patients and in human cultured cardiomyocytes

M Glenet
  • Fonction : Auteur
A Mirand
  • Fonction : Auteur
C Henquell
  • Fonction : Auteur
A-L Lebreil
F Berri
  • Fonction : Auteur
F Bani-Sadr
  • Fonction : Auteur
B Lina
  • Fonction : Auteur
I Schuffenecker
  • Fonction : Auteur
L Andreoletti
  • Fonction : Auteur
Marie‑laure Mathieu
  • Fonction : Auteur
Ellia Mezgueldi
  • Fonction : Auteur
Matthieu Verdan
  • Fonction : Auteur
Pascal Motreff
  • Fonction : Auteur
Samira Fafi-Kremer
Quentin Lepiller
Patrick Bruneva
  • Fonction : Auteur

Résumé

the french enterovirus Myocarditis Study Group (feMSG) * Major 5′terminally deleted (5′TD) group-B enterovirus (EV-B) populations were identified in heart biopsies of patients with fulminant myocarditis or dilated cardiomyopathy suggesting that these 5′TD forms are key drivers of host-cell interaction in EV cardiac infections. To date, early emergence of EV-B 5′TD forms and its impact on type 1 IFN response during acute myocarditis remains unknown. Using quantitative RACE-PCR assay, we identified major EV-B 5′tD RnA populations in plasma or heart samples of acute myocarditis cases. Deletions identified within the 5′ non-coding region of EV-B populations only affected secondary-structural elements of genomic RNA domain I and were distinguished in two major groups based on the extent of RnA structural deletions. proportions of these two respective EV-B 5′tD population groups were positively or negatively correlated with IFN-β levels in plasma samples of myocarditis patients. Transfection of synthetic CVB3/28 RNAs harboring various 5′terminal full-length or deleted sequences into human cultured cardiomyocytes demonstrated that viral genomic RnA domain i possessed essential immunomodulatory secondarystructural elements responsible for IFN-β pathway induction. Overall, our results highlight the early emergence of major EVB-TD populations which deletions affecting secondary-structures of RNA domain i can modulate innate immune sensing mechanisms in cardiomyocytes of patients with acute myocarditis.
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Dates et versions

hal-03267069 , version 1 (22-06-2021)

Identifiants

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M Glenet, Y N’guyen, A Mirand, C Henquell, A-L Lebreil, et al.. Major 5′terminally deleted enterovirus populations modulate type I IFN response in acute myocarditis patients and in human cultured cardiomyocytes. Scientific Reports, 2020, 10, ⟨10.1038/s41598-020-67648-5⟩. ⟨hal-03267069⟩

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