Investigation of squalene-doxorubicin distribution and interactions within single cancer cell using Raman microspectroscopy - Archive ouverte HAL Access content directly
Journal Articles Nanomedicine: Nanotechnology, Biology and Medicine Year : 2021

Investigation of squalene-doxorubicin distribution and interactions within single cancer cell using Raman microspectroscopy

Abstract

Intracellular distribution of doxorubicin (DOX) and its squalenoylated (SQ-DOX) nanoparticles (NPs) form in murine lung carcinoma M109 and human breast carcinoma MDA-MB-231 cells were investigated by Raman microspectroscopy. Pharmacological data showed that DOX induced higher cytotoxic effect than SQ-DOX NPs. Raman data were obtained using single-point measurements and imaging on the whole cell areas. These data showed that after DOX treatment at 1 µM, the spectral features of DOX were not detected in the M109 cell cytoplasm and nucleus. However, the intracellular distribution of SQ-DOX NPs was higher than DOX in the same conditions. In addition, SQ-DOX NPs were localized into both cell cytoplasm and nucleus. After 5 µM treatment, Raman bands of DOX at 1211 and 1241 cmwere detected in the nucleus. Moreover, the intensity ratio of these bands decreased, indicating DOX intercalation into DNA. However, after treatment with SQ-DOX NPs, the intensity of these Raman bands increased. Interestingly, with SQ-DOX NPs, the intensity of 1210/1241 cm-1 ratio was higher suggesting a lower fraction of intercalated DOX in DNA and higher amount of non-hydrolyzed SQ-DOX. Raman imaging data confirm this subcellular localization of these drugs in both M109 and MDA-MB-231 cells. These finding brings new insights to the cellular characterization of anticancer drugs at the molecular level, particularly in the field of nanomedicine.
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Dates and versions

hal-03406418 , version 1 (27-10-2021)

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Hassan Rammal, Almar Al Assaad, Franco Dosio, Barbara Stella, Andrei Maksimenko, et al.. Investigation of squalene-doxorubicin distribution and interactions within single cancer cell using Raman microspectroscopy. Nanomedicine: Nanotechnology, Biology and Medicine, 2021, 35, pp.102404. ⟨10.1016/j.nano.2021.102404⟩. ⟨hal-03406418⟩
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