Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle - Archive ouverte HAL Access content directly
Journal Articles Biomolecules Year : 2021

Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle

(1) , (2) , (3) , (4, 5) , (5) , (5) , (5) , (5) , (1) , (1)
1
2
3
4
5
Nicolas Berthélémy
Louis Danoux
  • Function : Author
Vincent Bardey
  • Function : Author
  • PersonId : 770745
  • IdRef : 152251766
Solène Mine
  • Function : Author
Christine Jeanmaire
  • Function : Author
  • PersonId : 767601
  • IdRef : 144370484

Abstract

The expression of glypicans in different hair follicle (HF) compartments and their potential roles during hair shaft growth are still poorly understood. Heparan sulfate proteoglycan (HSPG) distribution in HFs is classically investigated by conventional histology, biochemical analysis, and immunohistochemistry. In this report, a novel approach is proposed to assess hair histology and HSPG distribution changes in HFs at different phases of the hair growth cycle using infrared spectral imaging (IRSI). The distribution of HSPGs in HFs was probed by IRSI using the absorption region relevant to sulfation as a spectral marker. The findings were supported by Western immunoblotting and immunohistochemistry assays focusing on the glypican-1 expression and distribution in HFs. This study demonstrates the capacity of IRSI to identify the different HF tissue structures and to highlight protein, proteoglycan (PG), glycosaminoglycan (GAG), and sulfated GAG distribution in these structures. The comparison between anagen, catagen, and telogen phases shows the qualitative and/or quantitative evolution of GAGs as supported by Western immunoblotting. Thus, IRSI can simultaneously reveal the location of proteins, PGs, GAGs, and sulfated GAGs in HFs in a reagent- and label-free manner. From a dermatological point of view, IRSI shows its potential as a promising technique to study alopecia.
Fichier principal
Vignette du fichier
biomolecules-Colin Pierre 2021.pdf (40.31 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03370586 , version 1 (14-10-2021)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Charlie Colin-Pierre, Valérie Untereiner, Ganesh Sockalingum, Nicolas Berthélémy, Louis Danoux, et al.. Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle. Biomolecules, 2021, 11 (2), pp.192. ⟨10.3390/biom11020192⟩. ⟨hal-03370586⟩
49 View
19 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More