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Label-Free Infrared Spectral Histology of Skin Tissue Part II: Impact of a Lumican-Derived Peptide on Melanoma Growth

Abstract : Melanoma is the most aggressive type of cutaneous malignancies. In addition to its role as a regulator of extracellular matrix (ECM) integrity, lumican, a small leucine-rich proteoglycan, also exhibits anti-tumor properties in melanoma. This work focuses on the use of infrared spectral imaging (IRSI) and histopathology (IRSH) to study the effect of lumican-derived peptide (L9Mc) on B16F1 melanoma primary tumor growth. Female C57BL/6 mice were injected with B16F1 cells treated with L9Mc (n = 10) or its scrambled peptide (n = 8), and without peptide (control, n = 9). The melanoma primary tumors were subjected to histological and IR imaging analysis. In addition, immunohistochemical staining was performed using anti-Ki-67 and anti-cleaved caspase-3 antibodies. The IR images were analyzed by common K-means clustering to obtain high-contrast IRSH that allowed identifying different ECM tissue regions from the epidermis to the tumor area, which correlated well with H&E staining. Furthermore, IRSH showed good correlation with immunostaining data obtained with anti-Ki-67 and anti-cleaved caspase-3 antibodies, whereby the L9Mc peptide inhibited cell proliferation and increased strongly apoptosis of B16F1 cells in this mouse model of melanoma primary tumors.
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Contributor : Jean-Marc Ferez Connect in order to contact the contributor
Submitted on : Thursday, October 14, 2021 - 10:55:35 AM
Last modification on : Thursday, November 4, 2021 - 11:08:31 AM
Long-term archiving on: : Saturday, January 15, 2022 - 6:28:04 PM


fcell-08-00377(1) BREZILLON S ...
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Stéphane Brézillon, Valérie Untereiner, Hossam Taha Mohamed, Estelle Ahallal, Isabelle Proult, et al.. Label-Free Infrared Spectral Histology of Skin Tissue Part II: Impact of a Lumican-Derived Peptide on Melanoma Growth. Frontiers in Cell and Developmental Biology, Frontiers media, 2020, 8, ⟨10.3389/fcell.2020.00377⟩. ⟨hal-03370600⟩



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