Chemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell MCF7, but not in the triple-negative MDA-MB231 - Archive ouverte HAL Access content directly
Journal Articles Scientific Reports Year : 2017

Chemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell MCF7, but not in the triple-negative MDA-MB231

(1, 2) , (1, 2) , (1, 2) , (1, 2) , (3, 2)
1
2
3

Abstract

Autophagy is one of the chemotherapy resistance mechanisms in breast cancer. The aim of this study was to determine the level of recruitment of the autophagy pathway in the triple-negative breast cancer (TNBC) cell line MDA-MB231 compared with that in the control luminal breast cancer cell line MCF7 before and after treatment with chemotherapy drugs. Furthermore, we investigated the relationship between autophagy and EGFR, MUC1 and IL17-receptors as activators of autophagy. Immunohistochemistry was performed in cell culture blocks using LC3b, MUC1-C, EGFR, IL17A, IL17-RA and IL17-RB antibodies. We found that the basal autophagy level in MDA-MB231 was high, whereas it was low in MCF7. However, in contrast to MDA-MB231, the autophagy level was increased in MCF7 upon treatment with chemotherapy agents. Interestingly, we observed that the expression levels of MUC1-C, EGFR, IL17-RA, and IL17-RB were not modified by the same treatments. Furthermore, the chemotherapy treatments did not increase autophagy in TNBC cells without affecting the expression levels of MUC1-C, EGFR, IL17-RA or IL17-RB.
Fichier principal
Vignette du fichier
s41598-017-07489-x.pdf (2.14 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

hal-03424972 , version 1 (10-11-2021)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Christian Garbar, Corinne Mascaux, Jérôme Giustiniani, Yacine Merrouche, Armand Bensussan. Chemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell MCF7, but not in the triple-negative MDA-MB231. Scientific Reports, 2017, 7, ⟨10.1038/s41598-017-07489-x⟩. ⟨hal-03424972⟩
12 View
17 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More