Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS Study - Archive ouverte HAL Access content directly
Journal Articles Frontiers in Immunology Year : 2020

Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS Study

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Pauline Orquevaux
  • Function : Author
Gregory Gatouillat
  • Function : Author
Thierry Tabary
  • Function : Author
Marcelle Tonye Libyh
  • Function : Author
Jan Chrusciel
Moustapha Drame
  • Function : Author
Grace Stockton-Bliard
  • Function : Author
Zahir Amoura
  • Function : Author
Laurent Arnaud
Hanns-Martin Lorenz
  • Function : Author
Gilles Blaison
  • Function : Author
Bernard Bonnotte
Nadine Magy-Bertrand
  • Function : Author
Sabine Revuz
  • Function : Author
Reinhard Edmund Voll
  • Function : Author
Oliver Hinschberger
  • Function : Author
Andreas Schwarting
  • Function : Author
Bach Nga Pham
  • Function : Author
Thierry Martin
  • Function : Author
Jean-Loup Pennaforte
  • Function : Author

Abstract

and matched by age and gender. The serum from healthy voluntary blood donors was also tested. Anti-GBM were screened by fluorescence enzyme immunoassay (FEIA), and then by indirect immunofluorescence (IIF) in case of low reactivity detection (titer >6 U/ml). Results: The cohort was composed of 100 SLE patients with proliferative LN (27% with class III, 67% with class IV, and 6% with class V), compared to 100 SLE patients without LN and 100 controls. Patients were mostly Caucasian and met the ACR 1997 criteria and/ or the SLICC 2012 criteria. Among the 300 tested sera, no significant levels of anti-GBM antibodies were detected (>10 U/ml) by the automated technique, three sera were found "ambivalent" (>7 U/ml): one in the SLE with LN group and two in the SLE without LN group. Subsequent IIF assays did not detect anti-GBM antibodies. Conclusion: Anti-GBM antibodies were not detected in the serum of Caucasian patients with SLE, even in case of renal involvement, a situation favoring the antigenic exposure of glomerular basement membranes. Our results reaffirm the central role of anti-GBM antibodies as a specific diagnostic biomarker for Goodpasture vasculitis and therefore confirm that anti-GBM antibody must not be carried out in patients with SLE (with or without LN) in the absence of disease-suggestive symptoms.
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hal-03425005 , version 1 (10-11-2021)

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Nellie Bourse Chalvon, Pauline Orquevaux, Delphine Giusti, Gregory Gatouillat, Thierry Tabary, et al.. Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS Study. Frontiers in Immunology, 2020, 11, ⟨10.3389/fimmu.2020.597863⟩. ⟨hal-03425005⟩

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