Early Emergence of 5′ Terminally Deleted Coxsackievirus-B3 RNA Forms Is Associated with Acute and Persistent Infections in Mouse Target Tissues - Archive ouverte HAL Access content directly
Journal Articles Vaccines Year : 2022

Early Emergence of 5′ Terminally Deleted Coxsackievirus-B3 RNA Forms Is Associated with Acute and Persistent Infections in Mouse Target Tissues

(1, 2) , (2) , (3) , (4) , (5) , (2, 6) , (2)
1
2
3
4
5
6

Abstract

Major EV-B populations characterized by 5′ terminal deletions (5′TD) have been shown to be associated with the development of myocarditis and type 1 diabetes in mice or humans. To date, the dynamics of EV-B 5′TD-RNA forms’ emergence during the course of infection and their impact on cellular functions remain unclear. Using a RACE-PCR approach in CVB3/28-infected mouse organs, we showed an early (3 days post infection, DPI) emergence of major 5′TD populations associated with minor full-length RNA forms. Viral replication activities with infectious particle production were associated with heart, liver, and pancreas acute inflammatory lesions, whereas clearance of viral RNA without organ lesions was observed in the brain, lung, intestines, and muscles from 3 to 7 DPI. At 28 DPI, low viral RNA levels, +/-RNA ratios < 5 associated with viral protein 1 expression revealed a persistent infection in the heart and pancreas. This persistent infection was characterized by molecular detection of only 5′TD RNA forms that were associated with dystrophin cleavage in the heart and insulin production impairment in beta-pancreatic cells. These results demonstrated that major EV-B 5′TD RNA forms can be early selected during systemic infection and that their maintenance may drive EV-induced acute and persistent infections with target cell dysfunctions.
Fichier principal
Vignette du fichier
vaccines-10-01203-v2.pdf (2.4 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03758488 , version 1 (23-08-2022)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Domitille Callon, Anne-Laure Lebreil, Nicole Bouland, Caroline Fichel, Paul Fornès, et al.. Early Emergence of 5′ Terminally Deleted Coxsackievirus-B3 RNA Forms Is Associated with Acute and Persistent Infections in Mouse Target Tissues. Vaccines, 2022, 10 (8), pp.1203. ⟨10.3390/vaccines10081203⟩. ⟨hal-03758488⟩

Collections

URCA CARDIOVIR
16 View
7 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More