Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes - Archive ouverte HAL Access content directly
Journal Articles Journal of Biophotonics Year : 2019

Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes

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Ewelina Szafraniec
  • Function : Author
Edyta Kus
  • Function : Author
Adrianna Wislocka
  • Function : Author
Bozena Kukla
  • Function : Author
Ewa Sierka
  • Function : Author
Malgorzata Baranska
  • Function : Author
  • PersonId : 892829

Abstract

Liver sinusoidal endothelial cells (LSECs), a type of endothelial cells with unique morphology and function, play an important role in the liver hemostasis, and LSECs dysfunction is involved in the development of nonalcoholic fatty liver disease (NAFLD). Here, we employed Raman imaging and chemometric data analysis in order to characterize the presence of lipid droplets (LDs) and their lipid content in primary murine LSECs, in comparison with hepatocytes, isolated from mice on high-fat diet. On NAFLD development, LDs content in LSECs changed toward more unsaturated lipids, and this response was associated with an increased expression of stearylo-CoA desaturase-1. To the best of our knowledge, this is a first report characterizing LDs in LSECs, where their chemical composition is analyzed along the progression of NAFLD at the level of single LD using Raman imaging.
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Dates and versions

hal-02310339 , version 1 (10-10-2019)

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Ewelina Szafraniec, Edyta Kus, Adrianna Wislocka, Bozena Kukla, Ewa Sierka, et al.. Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes. Journal of Biophotonics, 2019, 12 (4), pp.e201800290. ⟨10.1002/jbio.201800290⟩. ⟨hal-02310339⟩
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