Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes - Université de Reims Champagne-Ardenne Accéder directement au contenu
Article Dans Une Revue Journal of Biophotonics Année : 2019

Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes

Ewelina Szafraniec
  • Fonction : Auteur
Edyta Kus
  • Fonction : Auteur
Adrianna Wislocka
  • Fonction : Auteur
Bozena Kukla
  • Fonction : Auteur
Ewa Sierka
  • Fonction : Auteur
Malgorzata Baranska
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  • PersonId : 892829

Résumé

Liver sinusoidal endothelial cells (LSECs), a type of endothelial cells with unique morphology and function, play an important role in the liver hemostasis, and LSECs dysfunction is involved in the development of nonalcoholic fatty liver disease (NAFLD). Here, we employed Raman imaging and chemometric data analysis in order to characterize the presence of lipid droplets (LDs) and their lipid content in primary murine LSECs, in comparison with hepatocytes, isolated from mice on high-fat diet. On NAFLD development, LDs content in LSECs changed toward more unsaturated lipids, and this response was associated with an increased expression of stearylo-CoA desaturase-1. To the best of our knowledge, this is a first report characterizing LDs in LSECs, where their chemical composition is analyzed along the progression of NAFLD at the level of single LD using Raman imaging.
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Dates et versions

hal-02310339 , version 1 (10-10-2019)

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Citer

Ewelina Szafraniec, Edyta Kus, Adrianna Wislocka, Bozena Kukla, Ewa Sierka, et al.. Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes. Journal of Biophotonics, 2019, 12 (4), pp.e201800290. ⟨10.1002/jbio.201800290⟩. ⟨hal-02310339⟩

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