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Article Dans Une Revue PLoS ONE Année : 2017

Buserelin alleviates chloride transport defect in human cystic fibrosis nasal epithelial cells

Résumé

Cystic fibrosis (CF) is the most common autosomal recessive disease in Caucasians caused by mutations in the gene encoding the Cystic Fibrosis Transmembrane conduc-tance Regulator (CFTR) chloride (Cl-) channel regulated by protein kinases, phosphatases, divalent cations and by protein-protein interactions. Among protein-protein interactions, we previously showed that Annexin A5 (AnxA5) binds to CFTR and is involved in the channel localization within membranes and in its Cl-channel function. The deletion of phenylalanine at position 508 (F508del) is the most common mutation in CF which leads to an altered protein (F508del-CFTR) folding with a nascent protein retained within the ER and is quickly degraded. We previously showed that AnxA5 binds to F508del-CFTR and that its increased expression due to a Gonadoliberin (GnRH) augments Cl-efflux in cells expressing F508del-CFTR. The aim of the present work was to use the GnRH analog buserelin which is already used in medicine. Human nasal epithelial cells from controls and CF patients (F508del/ F508del) were treated with buserelin and we show here that the treatment alleviates Cl-channel defects in CF cells. Using proteomics we highlighted some proteins explaining this result. Finally, we propose that buserelin is a potential new pharmaceutical compound that can be used in CF and that bronchus can be targeted since we show here that they express GnRH-R.
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Dates et versions

hal-02448639 , version 1 (26-05-2020)

Identifiants

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Marie-Laure Calvez, Nathalie Benz, Florentin Huguet, Aude Saint-Pierre, Elise Rouillé, et al.. Buserelin alleviates chloride transport defect in human cystic fibrosis nasal epithelial cells. PLoS ONE, 2017, 12 (11), pp.e0187774. ⟨10.1371/journal.pone.0187774⟩. ⟨hal-02448639⟩
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