The human NANOS3 gene contributes to lung tumour invasion by inducing epithelial-mesenchymal transition - Université de Reims Champagne-Ardenne
Journal Articles Journal of Pathology Year : 2015

The human NANOS3 gene contributes to lung tumour invasion by inducing epithelial-mesenchymal transition

Abstract

In this study, we explore the role of the human NANOS3 germline gene in lung tumour progression. We report that Nanos3 is overexpressed by invasive lung cancer cells and represents a new prognostic marker for non-small cell lung carcinomas (NSCLC). We also show that Nanos3 gene expression is restricted in testis and brain and is regulated by epigenetic events. Moreover, we demonstrate that Nanos3 is upregulated in cultured cells undergoing epithelialmesenchymal transition (EMT). Nanos3 overexpression in human NSCLC cell lines enhances their invasiveness by upregulating EMT, whereas its silencing induces mesenchymalepithelial transition. Nanos3 represses E-cadherin at the transcriptional level and upregulates Vimentin post-transcriptionally. Also, we show that Nanos3 binds mRNAs encoding Vimentin and regulates the length of the 3' poly(A) tail of VIM mRNA Finally, Nanos3 can also protect Vimentin mRNA from microRNA-mediated repression. We thus demonstrate a new role for Nanos3 in the acquisition of invasiveness by human lung tumour cells and propose a new mechanism of post-transcriptional regulation of EMT.
Fichier principal
Vignette du fichier
SimonGrelet_JPathol_2015.pdf (3.59 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-03007236 , version 1 (26-11-2020)

Identifiers

Cite

Simon Grelet, Vanessa Andries, Myriam Polette, Christine Gilles, Katrien Staes, et al.. The human NANOS3 gene contributes to lung tumour invasion by inducing epithelial-mesenchymal transition. Journal of Pathology, 2015, 237 (1), pp.25-37. ⟨10.1002/path.4549⟩. ⟨hal-03007236⟩

Collections

URCA P3CELL
46 View
188 Download

Altmetric

Share

More