Skip to Main content Skip to Navigation
Journal articles

Ticagrelor Prevents Endothelial Cell Apoptosis through the Adenosine Signalling Pathway in the Early Stages of Hypoxia

Abstract : Background: Several studies have reported the beneficial effects of anti-platelet drugs in cardioprotection against ischaemia-reperfusion injuries. To date, no studies have focused on the indirect cytoprotective effects of ticagrelor via adenosine receptor on the endothelium. Method: By evaluating cell viability and cleaved caspase 3 expression, we validated a model of endothelial cell apoptosis induced by hypoxia. In hypoxic endothelial cells treated with ticagrelor, we quantified the extracellular concentration of adenosine, and then we studied the involvement of adenosine pathways in the cytoprotective effect of ticagrelor. Results: Our results showed that 10 µM ticagrelor induced an anti-apoptotic effect in our model associated with an increase of extracellular adenosine concentration. Similar experiments were conducted with cangrelor but did not demonstrate an anti-apoptotic effect. We also found that A2B and A3 adenosine receptors were involved in the anti-apoptotic effect of ticagrelor in endothelial cells exposed to 2 h of hypoxia stress. Conclusion: we described an endothelial cytoprotective mechanism of ticagrelor against hypoxia stress, independent of blood elements. We highlighted a mechanism triggered mainly by the increased extracellular bioavailability of adenosine, which activates A2B and A3 receptors on the endothelium.
Document type :
Journal articles
Complete list of metadata

https://hal.univ-reims.fr/hal-03010123
Contributor : Jean-Marc Ferez <>
Submitted on : Tuesday, November 17, 2020 - 3:40:21 PM
Last modification on : Monday, April 19, 2021 - 8:53:25 AM
Long-term archiving on: : Thursday, February 18, 2021 - 7:49:55 PM

File

Ticagrelor Prevents Endothelia...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Catherine Feliu, Hélène Peyret, Sylvie Brassart-Pasco, Floriane Oszust, Gaël Poitevin, et al.. Ticagrelor Prevents Endothelial Cell Apoptosis through the Adenosine Signalling Pathway in the Early Stages of Hypoxia. Biomolecules, MDPI, 2020, 10 (5), pp.740. ⟨10.3390/BIOM10050740⟩. ⟨hal-03010123⟩

Share

Metrics

Record views

12

Files downloads

37