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Multicenter Evaluation of an ELISA for the Detection of Cryptosporidium spp. Antigen in Clinical Human Stool Samples

Abstract : Human cryptosporidiosis remains underdiagnosed, and rapid/accurate diagnosis is of clinical importance. Diagnosis of the Cryptosporidium oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, and requires skillful experience. Thus, we aimed to evaluate the usefulness of a coproantigen enzyme-linked immunosorbent assay (ELISA) test in detecting Cryptosporidium spp. from fecal specimens. For this aim, we evaluated the performances of a commercial ELISA (CoproELISA Cryptosporidium kit, Savyon Diagnostics, Israel) for the detection of Cryptosporidium spp. in random clinical stool samples through a multicenter study. The sensitivity and specificity for coproantigen ELISA were 98.86% and 94.32%, respectively. The coproantigen ELISA results indicate that the simple, rapid, reliable, and standardized immunoassay test is sensitive and specific for routine diagnosis, and may be useful for large-scale epidemiological studies of cryptosporidiosis.
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https://hal.univ-reims.fr/hal-03152175
Contributor : Laurence Dubois <>
Submitted on : Thursday, February 25, 2021 - 1:32:40 PM
Last modification on : Saturday, February 27, 2021 - 3:28:45 AM

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Romy Razakandrainibe, Célia Mérat, Nathalie Kapel, Marc Sautour, Karine Guyot, et al.. Multicenter Evaluation of an ELISA for the Detection of Cryptosporidium spp. Antigen in Clinical Human Stool Samples. Microorganisms, MDPI, 2021, 9 (2), pp.209. ⟨10.3390/microorganisms9020209⟩. ⟨hal-03152175⟩

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