Copper alloys surfaces have been proposed as a promising solution to avoid microbial contamination of surfaces but their antibiofilm effect is still unclear. All copper alloys tested have demonstrated efficient antibiofilm activity against Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, with differences between alloys. The artificial aging of copper alloys led to variable effects on the bacterial adhesion, depending on alloy. The results highlighted the promising antibiofilm activity of various copper alloys and suggest that the alloy composition is an essential parameter in this activity.