Effect of increased physical activity on growth and differentiation of regenerating rat soleus muscle - Université de Reims Champagne-Ardenne Accéder directement au contenu
Article Dans Une Revue European Journal of Applied Physiology Année : 1997

Effect of increased physical activity on growth and differentiation of regenerating rat soleus muscle

Philippe Noirez
Arnaud Ferry
Imed Ben Salah
  • Fonction : Auteur
Christine Le Page
  • Fonction : Auteur
Juan Pedro Wahrmann
  • Fonction : Auteur
Michel Rieu
  • Fonction : Auteur

Résumé

Our purpose was to determine the effect of physical exercise on growth and differentiation during regeneration of a slow-twitch muscle. Degeneration/regeneration of the left soleus muscles of Wistar female rats was induced by injection of a snake venom. Muscular differentiation was studied by monitoring the sequential expression of the various myosin heavy chain isoforms (MHCs). Rats were assigned to one of two groups: cage sedentary (n = 14) or exercised (n = 16). The exercise programme began 1-day post-injection and the rats ran 1 h/day on a motorized treadmill. Then, 9 and 25 days after venom treatment, the soleus MHC phenotype as determined by immunohistology, electrophoresis and immunoblotting, was studied. At 25 days the expression of MHCs by regenerating soleus was not changed by the increased level of physical activity (P  > 0.05). Exercised and sedentary regenerating muscles contained similar numbers of type-I fibres (100% of total fibres), levels of MHC-1 (85.4 and 89.5% of total MHCs), MHC-2a and M / HC-2x/d and their fibres expressed MHC-1 (100% of total fibres) and MHC-2 (45–50%) in the same way. Moreover, the masses of regenerating and nonregenerating soleus were significantly increased by physical exercise (P  < 0.02). At 9 days no effect of muscular exercise was found. In conclusion, endurance exercise did not alter differentiation of regenerating soleus. Moreover regenerating soleus can respond to increased physical activity by enhancing its mass in the same way as mature muscle.

Dates et versions

hal-03564778 , version 1 (10-02-2022)

Identifiants

Citer

Philippe Noirez, Arnaud Ferry, Imed Ben Salah, Christine Le Page, Juan Pedro Wahrmann, et al.. Effect of increased physical activity on growth and differentiation of regenerating rat soleus muscle. European Journal of Applied Physiology, 1997, 76 (3), pp.270-276. ⟨10.1007/s004210050247⟩. ⟨hal-03564778⟩
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