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Effect of hemolysis on Fourier transform infrared and Raman spectra of blood plasma

Abstract : Background: Atherosclerosis is an inflammatory process involving activation of monocytes recruited by various chemoattractant factors, among which lipoprotein(a) and its specific apolipoprotein apo(a). Lp(a) contains a specific apolipoprotein apo(a) which size is determined by a variable number of repeats of a specific structural domain, the kringle IV type 2 (IV-2). Lp(a) plasma concentration and apo(a) size is inversely correlated, and smaller apo(a) are major risk factors for coronary heart disease. Design and methods: The aim of this study was to evaluate the effect of recombinant apo(a) isoforms (containing 10, 18 or 34 kringles) on monocytes interacting with type I collagen. Results: Apo(a) isoforms stimulated reactive oxygen species (ROS) and matrix metalloproteinase-9 (MMP-9) production by monocytes, and not modified monocytes adhesion on type I collagen. This effect was specific of apo(a) since no effect was observed in the presence of plasminogen and was inversely related to apo(a) size. The lysine analogue 6-aminohexanoic acid which blocks the lysine binding sites (LBS), and carboxypeptidase B (CpB) which cleaves carboxy-terminal lysine residues, abolished apo(a)-induced ROS and MMP-9 production, highlighting an effect mediated by apo(a) lysing-binding sites. Conclusions: These results indicate that activation of collagen-primed monocytes stimulated with apo(a) is a Kringle number-dependent effect and reinforce the hypothesis of a role for small size apo(a) isoforms in atherothrombosis.
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Contributor : Brigitte PERRON Connect in order to contact the contributor
Submitted on : Tuesday, July 12, 2022 - 2:59:44 PM
Last modification on : Friday, August 5, 2022 - 11:23:21 AM

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Dinesh Medipally, Daniel Cullen, Valérie Untereiner, Jane Bryant, Ganesh Sockalingum, et al.. Effect of hemolysis on Fourier transform infrared and Raman spectra of blood plasma. Journal of Biophotonics, Wiley, 2020, 13, ⟨10.1002/jbio.201960173⟩. ⟨hal-03590728⟩



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