In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging - Université de Reims Champagne-Ardenne
Article Dans Une Revue International Journal of Molecular Sciences Année : 2022

In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging

Cecilia Virginia Gheran
  • Fonction : Auteur
Sorina Nicoleta Voicu
Bianca Galateanu
Cyril Cadiou
Anca Dinischiotu
  • Fonction : Auteur

Résumé

The aim of this study was to investigate the biocompatibility of contrast agents, such as gadolinium 1, 4, 7, 10 tetraazacyclo-dodecane tetraacetic acid (GdDOTA) and gadolinium dioctyl terephthalate (GdDOTP), encapsulated in a polymeric matrix containing chitosan and hyaluronic acid using RAW264.7 murine macrophages and human blood samples. The cell viability and cytotoxicity were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays, while cell cycle analysis was determined in RAW264.7 cells using flow cytometry. The mitochondrial membrane potential (MMP), hemolytic index, complement activation, and thrombogenic potential of gadolinium (Gd) containing nanohydrogels were measured by fluorometric and spectrophotometric methods. Taken together, our results demonstrate the good bio- and hemocompatibility of chitosan-based nanohydrogels with the RAW264.7 cell line and human blood cells, suggesting that these could be used as injectable formulations for the magnetic resonance imaging diagnostic of lymph nodes.

Domaines

Chimie

Dates et versions

hal-03614974 , version 1 (21-03-2022)

Identifiants

Citer

Cecilia Virginia Gheran, Sorina Nicoleta Voicu, Bianca Galateanu, Maité Callewaert, Juliette Moreau, et al.. In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging. International Journal of Molecular Sciences, 2022, 23 (6), pp.3258. ⟨10.3390/ijms23063258⟩. ⟨hal-03614974⟩
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