HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Synthesis and Characterization of Conjugated Hyaluronic Acids. Application to Stability Studies of Chitosan-Hyaluronic Acid Nanogels Based on Fluorescence Resonance Energy Transfer

Abstract : Hyaluronic acid (HA) was functionalized with a series of amino synthons (octylamine, polyethylene glycol amine, trifluoropropyl amine, rhodamine). Sodium hyaluronate (HAs) was first converted into its protonated form (HAp) and the reaction was conducted in DMSO by varying the initial ratio (−NH2 (synthon)/COOH (HAp)). HA derivatives were characterized by a combination of techniques (FTIR, 1H NMR, 1D diffusion-filtered 19F NMR, DOSY experiments), and degrees of substitution (DSHA) varying from 0.3% to 47% were determined, according to the grafted synthon. Nanohydrogels were then obtained by ionic gelation between functionalized hyaluronic acids and chitosan (CS) and tripolyphosphate (TPP) as a cross-linker. Nanohydrogels for which HA and CS were respectively labeled by rhodamine and fluorescein which are a fluorescent donor-acceptor pair were subjected to FRET experiments to evaluate the stability of these nano-assemblies.
Document type :
Journal articles
Complete list of metadata

https://hal.univ-reims.fr/hal-03614986
Contributor : Cadiou Cyril Connect in order to contact the contributor
Submitted on : Monday, March 21, 2022 - 11:26:33 AM
Last modification on : Tuesday, March 22, 2022 - 3:35:19 AM

Links full text

Identifiers

Collections

Citation

Volodymyr Malytskyi, Juliette Moreau, Maité Callewaert, Céline Henoumont, Cyril Cadiou, et al.. Synthesis and Characterization of Conjugated Hyaluronic Acids. Application to Stability Studies of Chitosan-Hyaluronic Acid Nanogels Based on Fluorescence Resonance Energy Transfer. Gels, MDPI, 2022, 8 (3), pp.182. ⟨10.3390/gels8030182⟩. ⟨hal-03614986⟩

Share

Metrics

Record views

12