Contribution of the anaerobic blood culture vial for the recovery of Candida glabrata : A retrospective multicentric study - Université de Reims Champagne-Ardenne
Journal Articles Medical Mycology Year : 2022

Contribution of the anaerobic blood culture vial for the recovery of Candida glabrata : A retrospective multicentric study

Eric Farfour
Marie-Fleur Durieux
  • Function : Author
Vincent Fihman
Françoise Botterel
Marie-Emmanuelle Juvin
  • Function : Author
Paul-Louis Woerther
  • Function : Author
Francoise Foulet
  • Function : Author
Nawel Ait Ammar
  • Function : Author
Patrice Le Pape
Isabelle Villena
  • Function : Author
Christophe Des Champs
  • Function : Author
Laurent Mereghetti
  • Function : Author
Guillaume Desoubeaux
  • Function : Author
Daniel Ajzenberg
  • Function : Author
Benjamin Maneglier
  • Function : Author

Abstract

Abstract Although Candida spp are aerobic microorganisms, some Candida strains, mainly Candida glabrata, can be recovered from anaerobic blood culture vials. We assessed the contribution of the anaerobic vials for the diagnosis of candidemia, especially for C. glabrata. We conducted a multicenter retrospective study including eight university or regional hospitals. A single episode of monomicrobial candidemia per patient was included from September 1st, 2016, to August 31st, 2019. The characteristics of all aerobic and anaerobic blood culture vials sampled within 2 h before and after the first positive blood culture vials were recorded (type of vials, result, and for positive vials time-to-positivity and Candida species). Overall, 509 episodes of candidemia were included. The main species were C. albicans (55.6%) followed by C. glabrata (17.1%), C. parapsilosis (4.9%), and C. tropicalis (4.5%). An anaerobic vial was positive in 76 (14.9%) of all episodes of which 56 (73.8%) were due to C. glabrata. The number of C. glabrata infections only positive in anaerobic vials was 1 (2.6%), 1 (11.1%), and 15 (37.5%) with the BACT/ALERT 3D the BACT/ALERT VIRTUO and the BACTEC FX instrument, respectively (P < 0.01). The initial positivity of an anaerobic vial was highly predictive of the isolation of C. glabrata with the BACTEC FX (sensitivity of 96.8%). C. glabrata time-to-positivity was shorter in anaerobic vial than aerobic vial with all instruments. Anaerobic blood culture vials improve the recovery of Candida spp mainly C. glabrata. This study could be completed by further analyses including mycological and pediatric vials. Lay summary Although Candida spp are aerobic microorganisms, C. glabrata is able to grow in anaerobic conditions. In blood culture, the time-to-positivity of C. glabrata is shorter in anaerobic than aerobic vials. Only the anaerobic vial was positive in up to 15 (37.5%) C. glabrata bloodstream infections.
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Dates and versions

hal-04316567 , version 1 (30-11-2023)

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Eric Farfour, Cecile Le Brun, Assaf Mizrahi, Pauline Bargain, Marie-Fleur Durieux, et al.. Contribution of the anaerobic blood culture vial for the recovery of Candida glabrata : A retrospective multicentric study. Medical Mycology, 2022, 60 (4), ⟨10.1093/mmy/myac021⟩. ⟨hal-04316567⟩
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