Contribution of the anaerobic blood culture vial for the recovery of Candida glabrata : a retrospective multicentric study - Archive ouverte HAL Access content directly
Journal Articles Medical Mycology Year : 2022

Contribution of the anaerobic blood culture vial for the recovery of Candida glabrata : a retrospective multicentric study

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Eric Farfour
Cecile Le Brun
Assaf Mizrahi
  • Function : Author
Pauline Bargain
  • Function : Author
Marie-Fleur Durieux
  • Function : Author
Frédérique Boquel
  • Function : Author
Stéphane Corvec
  • Function : Author
Fakhri Jeddi
  • Function : Author
Antoine Huguenin
  • Function : Author
Olivier Barraud
  • Function : Author
Marlène Amara
  • Function : Author
Vincent Fihman
Eric Bailly
  • Function : Author
Françoise Botterel
Marc Vasse
  • Function : Author

Abstract

Abstract Although Candida spp are aerobic microorganisms, some Candida strains, mainly Candida glabrata, can be recovered from anaerobic blood culture vials. We assessed the contribution of the anaerobic vials for the diagnosis of candidemia, especially for C. glabrata. We conducted a multicenter retrospective study including eight University or regional hospitals. A single episode of monomicrobial candidemia per patient was included from September 1st, 2016, to August 31st, 2019. The characteristics of all aerobic and anaerobic blood culture vials sampled within 2 hours before and after the first positive blood culture vials were recorded (type of vials, result, and for positive vials time-to-positivity and Candida species). Overall, 509 episodes of candidemia were included. The main species were C. albicans (55.6%) followed by C. glabrata (17.1%), C. parapsilosis (4.9%), and C. tropicalis (4.5%). An anaerobic vial was positive in 76 (14.9%) of all episodes of which 56 (73.8%) were due to C. glabrata. The number of C. glabrata infections only positive in anaerobic vials was 1 (2.6%), 1 (11.1%), and 15 (37.5%) with the BACT/ALERT 3D the BACT/ALERT VIRTUO and the BACTEC FX instrument respectively (P < 0.01). The initial positivity of an anaerobic vial was highly predictive of the isolation of C. glabrata with the BACTEC FX (sensitivity of 96.8%). C. glabrata time-to-positivity was shorter in anaerobic vial than aerobic vial with all instruments. Anaerobic blood culture vials improve the recovery of Candida spp mainly C. glabrata. This study could be completed by further analyzes including mycological and pediatric vials.
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Dates and versions

hal-03647048 , version 1 (20-04-2022)

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Eric Farfour, Cecile Le Brun, Assaf Mizrahi, Pauline Bargain, Marie-Fleur Durieux, et al.. Contribution of the anaerobic blood culture vial for the recovery of Candida glabrata : a retrospective multicentric study. Medical Mycology, 2022, ⟨10.1093/mmy/myac021⟩. ⟨hal-03647048⟩

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