Contribution of the anaerobic blood culture vial for the recovery of Candida glabrata : A retrospective multicentric study - Université de Reims Champagne-Ardenne
Journal Articles Medical Mycology Year : 2022

Contribution of the anaerobic blood culture vial for the recovery of Candida glabrata : A retrospective multicentric study

Eric Farfour (1) , Cecile Le Brun (2) , Assaf Mizrahi (3, 4) , Pauline Bargain (5) , Marie-Fleur Durieux (6) , Frédérique Boquel (7) , Stéphane Corvec (8) , Fakhri Jeddi (9) , Anaëlle Muggeo (10, 11) , Antoine Huguenin (12) , Olivier Barraud (6, 13, 14) , Marlène Amara (15) , Vincent Fihman (7) , Eric Bailly (16) , Françoise Botterel (17) , Thomas Guillard (10, 11) , Marc Vasse (1, 18) , Aurélie Guillouzouic (19, 20) , Louise Ruffier d'Epenoux (19, 20) , Pascale Bémer (19, 20) , Marie-Emmanuelle Juvin , Paul-Louis Woerther , Francoise Foulet , Nawel Ait Ammar , Patrice Le Pape , Isabelle Villena , Christophe Des Champs , Laurent Mereghetti , Guillaume Desoubeaux , Alban Le Monnier (4, 21, 22) , Daniel Ajzenberg , Benjamin Maneglier
1 Hôpital Foch [Suresnes]
2 Service de Bactériologie-Virologie-Hygiène [Tours]
3 Centre hospitalier Saint-Joseph [Paris]
4 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
5 Hopital A.-Mignot
6 CHU Limoges
7 CHU Henri Mondor [Créteil]
8 INCIT - Immunology and New Concepts in ImmunoTherapy
9 IICiMed - Cibles et Médicaments des Infections et de l'Immunité
10 P3CELL - Pathologies Pulmonaires et Plasticité Cellulaire - UMR-S 1250
11 CHU Reims - Hôpital universitaire Robert Debré [Reims]
12 ESCAPE - Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle
13 CIC1435 - Centre d'Investigation Clinique de Limoges
14 RESINFIT - Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques
15 CHV - Centre Hospitalier de Versailles André Mignot
16 CHU Trousseau [Tours]
17 DYNAMIC - Dynamic Microbiology - EA 7380
18 HITH - U1176 Inserm - CHU Bicêtre - Hémostase, Inflammation, Thrombose
19 CHU Nantes - Centre Hospitalier Universitaire de Nantes = Nantes University Hospital
20 Hôtel-Dieu de Nantes
21 Université Paris-Saclay
22 hpsj - Groupe Hospitalier Paris Saint-Joseph
Eric Farfour
Hôpital Foch [Suresnes]
Cecile Le Brun
Service de Bactériologie-Virologie-Hygiène [Tours]
Assaf Mizrahi
  • Function : Author
Centre hospitalier Saint-Joseph [Paris]
MICrobiologie de l'ALImentation au Service de la Santé
Pauline Bargain
  • Function : Author
Hopital A.-Mignot
Marie-Fleur Durieux
  • Function : Author
CHU Limoges
Frédérique Boquel
  • Function : Author
CHU Henri Mondor [Créteil]
Stéphane Corvec
  • Function : Author
Immunology and New Concepts in ImmunoTherapy
Fakhri Jeddi
  • Function : Author
Cibles et Médicaments des Infections et de l'Immunité
Anaëlle Muggeo
  • Function : Author
Pathologies Pulmonaires et Plasticité Cellulaire - UMR-S 1250
Hôpital universitaire Robert Debré [Reims]
Antoine Huguenin
  • Function : Author
Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle
Olivier Barraud
CHU Limoges
Centre d'Investigation Clinique de Limoges
Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques
Marlène Amara
  • Function : Author
Centre Hospitalier de Versailles André Mignot
Vincent Fihman
CHU Henri Mondor [Créteil]
Eric Bailly
  • Function : Author
CHU Trousseau [Tours]
Françoise Botterel
Dynamic Microbiology - EA 7380
Thomas Guillard
Pathologies Pulmonaires et Plasticité Cellulaire - UMR-S 1250
Hôpital universitaire Robert Debré [Reims]
Marc Vasse
  • Function : Author
Hôpital Foch [Suresnes]
Hémostase, Inflammation, Thrombose
Aurélie Guillouzouic
  • Function : Author
Centre Hospitalier Universitaire de Nantes = Nantes University Hospital
Hôtel-Dieu de Nantes
Louise Ruffier d'Epenoux
  • Function : Author
Centre Hospitalier Universitaire de Nantes = Nantes University Hospital
Hôtel-Dieu de Nantes
Pascale Bémer
  • Function : Author
Centre Hospitalier Universitaire de Nantes = Nantes University Hospital
Hôtel-Dieu de Nantes
Marie-Emmanuelle Juvin
  • Function : Author
Paul-Louis Woerther
  • Function : Author
Francoise Foulet
  • Function : Author
Nawel Ait Ammar
  • Function : Author
Patrice Le Pape
Isabelle Villena
  • Function : Author
Christophe Des Champs
  • Function : Author
Laurent Mereghetti
  • Function : Author
Guillaume Desoubeaux
  • Function : Author
Alban Le Monnier
MICrobiologie de l'ALImentation au Service de la Santé
Université Paris-Saclay
Groupe Hospitalier Paris Saint-Joseph
CV
Daniel Ajzenberg
  • Function : Author
Benjamin Maneglier
  • Function : Author

Abstract

Abstract Although Candida spp are aerobic microorganisms, some Candida strains, mainly Candida glabrata, can be recovered from anaerobic blood culture vials. We assessed the contribution of the anaerobic vials for the diagnosis of candidemia, especially for C. glabrata. We conducted a multicenter retrospective study including eight university or regional hospitals. A single episode of monomicrobial candidemia per patient was included from September 1st, 2016, to August 31st, 2019. The characteristics of all aerobic and anaerobic blood culture vials sampled within 2 h before and after the first positive blood culture vials were recorded (type of vials, result, and for positive vials time-to-positivity and Candida species). Overall, 509 episodes of candidemia were included. The main species were C. albicans (55.6%) followed by C. glabrata (17.1%), C. parapsilosis (4.9%), and C. tropicalis (4.5%). An anaerobic vial was positive in 76 (14.9%) of all episodes of which 56 (73.8%) were due to C. glabrata. The number of C. glabrata infections only positive in anaerobic vials was 1 (2.6%), 1 (11.1%), and 15 (37.5%) with the BACT/ALERT 3D the BACT/ALERT VIRTUO and the BACTEC FX instrument, respectively (P < 0.01). The initial positivity of an anaerobic vial was highly predictive of the isolation of C. glabrata with the BACTEC FX (sensitivity of 96.8%). C. glabrata time-to-positivity was shorter in anaerobic vial than aerobic vial with all instruments. Anaerobic blood culture vials improve the recovery of Candida spp mainly C. glabrata. This study could be completed by further analyses including mycological and pediatric vials. Lay summary Although Candida spp are aerobic microorganisms, C. glabrata is able to grow in anaerobic conditions. In blood culture, the time-to-positivity of C. glabrata is shorter in anaerobic than aerobic vials. Only the anaerobic vial was positive in up to 15 (37.5%) C. glabrata bloodstream infections.

Domains

Health Infectious diseases Bacteriology Virology Santé publique et épidémiologie

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https://hal.science/hal-04316567

Submitted on : Thursday, November 30, 2023-5:19:55 PM

Last modification on : Monday, September 16, 2024-1:30:05 PM

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hal-04316567 , version 1 (30-11-2023)

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Eric Farfour, Cecile Le Brun, Assaf Mizrahi, Pauline Bargain, Marie-Fleur Durieux, et al.. Contribution of the anaerobic blood culture vial for the recovery of Candida glabrata : A retrospective multicentric study. Medical Mycology, 2022, 60 (4), ⟨10.1093/mmy/myac021⟩. ⟨hal-04316567⟩

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UNILIM ANSES AGROPARISTECH CNRS UNIV-ANGERS URCA UPEC GEIST COMUE-NORMANDIE UNIV-PARIS-SACLAY P3CELL ESCAPE-REIMS UNIROUEN ESCAPE-ROUEN ENVA INRAE DYNAMIC GS-BIOSPHERA GS-LIFE-SCIENCES-HEALTH GS-HEALTH-DRUG-SCIENCES INCIT MICA-UNITES MICALIS OMEGAHEALTH IICIMED NANTES-UNIVERSITE LABCIS HITH BAPS-MICALIS UFR-MEDECINE-NANTES
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